I actually failed my molecular biology course, and I’m still a little salty about that. I understood molecular biology. I didn’t memorize stuff like the order in which subunits bind to assemble the pre-replication complex.
After ORC1-6 bind the origin of replication, Cdc6 is recruited. Cdc6 recruits the licensing factor Cdt1 and MCM2-7. Cdt1 binding and ATP hydrolysis by the ORC and Cdc6 load MCM2-7 onto DNA.
Note that they’re numbered but the numbers aren’t related to the order in which they act. I don’t need to know that. No one who doesn’t do research specifically on the pre-replication complex needs to know that.
I actually failed my molecular biology course, and I’m still a little salty about that. I understood molecular biology. I didn’t memorize stuff like the order in which subunits bind to assemble the pre-replication complex.
Note that they’re numbered but the numbers aren’t related to the order in which they act. I don’t need to know that. No one who doesn’t do research specifically on the pre-replication complex needs to know that.
(Excuses, excuses…)